Flatten the layers and save the greyscale image as a {something}-bw.tif
Use go back or undo and turn off the greyscale layer and flatten again. Save the coloured layer as {something}-col.tif
We've finished with photoshop. There are several software packages out there for creating 3D. I use two, both authored in Russia. The software is for creating Lenticular images, you know - those 3d covers you see on posters, dvds, and advertising displays. For the swivelling 3D images I use Stereo Tracer. It's not cheap but I think it's the easist to use and the effects are very good. if you know of cheaper ones, drop me a line.
You open your colour and greyscale image with the software, and generate frames (15 is a good number) after setting parallax, setting zero parallax, and number of frames. Use 5, 6, or 7 for parallax, and 250 for zero parallax. You can experiment with these to determine the best effect. I have done all this to Dennis's bedbug image. See below...
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