Page 7 - Demo
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Step 5: Adding fructose mountant Using the non-brush end of your clean paint brush, dip the tip gently into the jar of fructose%u2014[1]. Remove the brush and lower it close to the stained solution on the slide. Let one or two drops drip down onto the water and stain%u2014[2], then gently stir with the same end of your brush. Wash the brush, both ends afterwards so it doesn%u2019t get fouled up for later use. Step 6: Applying the cover slip Breathe onto the coverslip to moisten it (helping to minimise any air getting trapped), then position and drop it onto the slide as previously learned%u2014[3]. Make sure the coverslip is square to the slide, and positioned correctly over the well containing the mix of cheek cells. Use tweezers against the coverslip edge to gently slide the slip until square with the %u2018well%u2019 you made%u2014[4]. Step 7: Fixing the corners Use kitchen towel or tissue to clean up any spillage, being careful not to dislodge the coverslip. Use a water filled brush either side of the slip to help wash away the stain. Leave the slide somewhere warm for an hour or two so the edges dry out a little. Then use nail varnish and apply a drop to each corner of the slip so that it goes from the corner and onto the slide%u2014[5].Step 8: Seal the cover slip Leave the slide somewhere warm for 15 minutes or more so the varnish dries, locking the slip to the glass. Paint the edge of the cover slip with nail varnish, ensuring the slip and glass slide are both covered at each edge to seal the coverslip. Work fast so the new varnish does not %u2018un-stick%u2019 the corners, which it might do as the additional varnish solvent works on the four dots%u2014[6]. 1 Step 8: Optional%u2014paint with lacquer Another option is you can paint over the varnish with lacquer or enamel paint (red or black is best) to strengthen the seal. It%u2019s worth doing if you want to keep the slide for longer than a few months. Purchased from Amazon but also sold in Airfix or Revell model shops. Tiny can. Other enamels will do as well. 2 3 4 5 6 22
