Fluorescence Microscopy

Gregor Overney, California, USA
 

Leitz Ortholux II with Ploemopak 2.2 (1975)

Introduction

Fluorescence microscopy is the use of a microscope to excite fluorescent molecules and use the emission wavelengths to form an image. This method allows for the precise identification and localization of specific structures, as well as the investigation of processes within cells.

This puplication consists of two chapters. The first chapter is a short introduction to Fluorescence Microscopy. In the second chapter, we will discuss some microscopes used for Fluorescence Microscopy. Although we are mainly focusing on older Leitz instruments, the basic principles are the same even for today's research systems. The links to PDF files of these two chapters are listed below.

Chapters

    [1] Fluorescence Microscopy - A Short Introduction

    [2] Fluorescence Microscopy - Instrumentation

     



 

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© Gregor Overney

First published in the March 2025 edition of Micscape Magazine.

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